A research paper submitted in partial fulfillment of the requirements for the degree of Master of Arts for Teachers in the Field of Biological Sciences. University of Washington

Committee Chair
Dr. Kenneth Mackie

Abstract

Genetic instructions for the cannabinoid receptor rCB1, a G protein-coupled receptor found typically on neuronal cell membranes, can have a preprolactin signal sequence (pplss) attached to its amino terminus for the purpose of enhancing translocation to the cell surface. Once the rCB1 molecule is synthesized on the surface of the ER, the pplss is instrumental in initiating the process of translocation, perhaps via Signal Recognition Particles (SRPs). Human Embryonic Kidney (HEK293) cells were transfected with a DNA construct containing the instructions for rCB1 production as well as a pplss. This resulted in a successful translocation of rCB1 to the cell membrane.