| Parichy Lab |
Protocols
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| In situ hybridization of whole-mount larval zebrafish. This protocol extends standard embryo in situ protocols for use at metamorphic stages (10–28 dpf). The protocol is still under development and is updated periodically. Last update: 26 June 2007. | |
| In situ hybridization on sections of larval zebrafish. For detecting mRNA on cryosections of larval zebrafish. | |
| Clearing and staining for bone and cartilage in larval fish. An alcian blue and alizarin red clearing and staining procedure modifed for use with larval zebrafish. | |
| Gateway and Tol2 transgenesis. A protocol for using Gateway vectors and the Tol2 transposable element system for generating transgenic fish. | |
| Stock maintenance and breeding methods. A protocol for how we maintain our stocks of zebrafish and other species by selective breeding and how we perform natural spawnings and in vitro fertilization. Added 26 February 2008. | |
| High density rotifer culture and feeding. A protocol for growing marine rotifers used as a food source for young zebrafish larvae at high density in Aquatic Ecosystems rotifer culture systems. Updated 30 November 2008. More information. | |
| High density rotifer culture daily checklist. For logging rotifer health and system parameters. | |
| Marine rotifer culture and feeding. A protocol for growing marine rotifers used as a food source for young zebrafish larvae. Updated 20 November 2005, but no longer used by lab (see above). | |
| Fish maintenance. Standard operating procedure (SOP) for zebrafish feeding, health evaluation, and system checks. Updated 13 November 2008. | |
| Fish and rotifer maintenance logs. For daily recording of fish feeding, health, and system parameters. 1=main system; 2=stand-alone racks; 3=quarantine room, 4=embryo incubator, 5=rotifer log. | |
| Record keeping. SOP for recording zebrafish stocks and tank labeling. Updated 21 November 2008. | |
| Embryo and larva rearing. SOP for zebrafish embryo and larval rearing. Updated 13 November 2008. | |
| Recipes. For fish foods, water, etc. | |
| Quarantine room. SOP for zebrafish embryo and larval rearing | |
| Post-embryonic staging. Normal table of zebrafish development after hatching (from Developmental Dynamics) | |
| Development time – temperature converter. There is a static pdf version and a dynamic Excel version. For each column, you can change the temperature by changing the top cell and you can put in a specific number of hours or days by changing the left columns, or by typing in the white cells at the upper left. You can also use this to estimate hours until a particular stage is reached (e.g., 5 hours to go at 28.5 C is equivalent to 6.7 hours to go at 24 C). The table is based on Chuck Kimmel's estimated linear function in embryos and may or may not be accurate at later stages. |