Parichy Lab
Images: salamanders

 

Salamander development, genetics, and genomics

In addition to zebrafish and their relatives, the lab maintains an interest in salamander pigment pattern development and collaborates on studies of salamander genetics and genomics.

 

embryos

Shown are embryos of Taricha torosa (upper) and Ambystoma tigrinum (lower) at the tailbud stages. Because of their large size (~5 mm), salamander embryos are ideal for experimental manipulation.

larvae

Larvae of three species of Ambystoma that differ in pigment pattern. These patterns are especially distinctive at the hatching stage seen here, when larvae are especially vulnerable to predators.

axolotls

Axolotls, Ambystoma mexicanum, do not undergo metamorphosis and instead remain in a permanently aquatic, paedomorphic state.  Shown is a wild-type adult as well as a white mutant adult that completely lacks pigment cells due to an early defect in neural crest development.

Ambystoma tigrinum

This species exhibits an early pigment pattern in aquatic larvae consisting of a light horizontal stripe in the middle of the flank and vertical bars of melanocytes over the dorsal flank. Subsequently, the larvae metamorphose and develop a terrestrial adult pigment pattern of bright spots on a dark background.

Taricha torosa

This species exhibits horizontal melanophore stripes at early larval stages and a uniform brown pattern as a terrestrial adult.  Experimental analyses reveal evolutionarily derived patterning mechanisms, as compared to other salamander species.

Ambystoma species

Terrestrial adults of six species of Ambystoma exhibiting a variety of pigment patterns including rings, speckling, longitudinal stripes and irregular spots.